Table Of ContentPATCH CLAMP TECHNIQUE
Edited by Fatima Shad Kaneez
Patch Clamp Technique
Edited by Fatima Shad Kaneez
Published by InTech
Janeza Trdine 9, 51000 Rijeka, Croatia
Copyright © 2012 InTech
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First published March, 2012
Printed in Croatia
A free online edition of this book is available at www.intechopen.com
Additional hard copies can be obtained from [email protected]
Patch Clamp Technique, Edited by Fatima Shad Kaneez
p. cm.
ISBN 978-953-51-0406-3
Contents
Preface IX
Part 1 Exploring Cellular Mechanisms
Using Patch Clamp Technique 1
Chapter 1 Patch ClampTechnique for Looking at
Serotonin Receptors in B103 Cell Lines: A Black Box Test 3
K. Fatima-Shad and K. Bradley
Chapter 2 Intracellular Signaling Pathways Integrating the Pore
Associated with P2X7R Receptor with Other Large Pores 37
L.G.B. Ferreira, R.A.M. Reis, L.A. Alves and R.X. Faria
Chapter 3 Patch Clamp Study of
Neurotransmission at Single Mammalian CNS Synapses 55
Norio Akaike
Chapter 4 Single-Channel Properties and
Pharmacological Characteristics of
K Channels in Primary Afferent Neurons 73
ATP
Takashi Kawano
Chapter 5 Regulation of Renal Potassium
Channels by Protein Kinases and Phosphatases 91
Manabu Kubokawa
Chapter 6 BK Channels – Focus on Polyamines,
Ethanol/Acetaldehyde and Hydrogen Sulfide (H S) 109
2
Anton Hermann, Guzel F. Sitdikova and Thomas M. Weiger
Chapter 7 From Action Potential-Clamp to "Onion-Peeling"
Technique – Recording of Ionic Currents Under
Physiological Conditions 143
Ye Chen-Izu, Leighton T. Izu, Peter P. Nanasi and Tamas Banyasz
Chapter 8 Electrophysiological Techniques for
Mitochondrial Channels 163
Rainer Schindl and Julian Weghuber
VI Contents
Chapter 9 Patch-Clamp Analysis of
Membrane Transport in Erythrocytes 171
Guillaume Bouyer, Serge Thomas and Stéphane Egée
Chapter 10 Electrical Membrane Properties in
the Model Leishmania-Macrophage 203
Marcela Camacho
Part 2 Advantages of Using Patch Clamp Technique 231
Chapter 11 Cardiac Channelopathies:
Disease at the Tip of a Patch Electrode 233
Brian P. Delisle
Chapter 12 Gating Charge Movement in Native Cells:
Another Application of the Patch Clamp Technique 255
Oscar Vivas, Isabel Arenas and David E. García
Chapter 13 Use of Patch Clamp Electrophysiology to
Identify Off-Target Effects of Clinically Used Drugs 267
Lioubov I. Brueggemann and Kenneth L. Byron
Chapter 14 Role of Non-Steroidal Anti-Inflammatory Drugs (NSAIDs) in
Modulating Vascular Smooth Muscle Cells by Activating
Large-Conductance Potassium Ion Channels 283
Donald K. Martin, Christopher G. Schyvens, Kenneth R. Wyse,
Jane A. Bursill, Robert A. Owe-Young,
Peter S. Macdonald and Terence J. Campbell
Chapter 15 Perforated Patch Clamp in Non-Neuronal Cells,
the Model of Mammalian Sperm Cells 301
Jorge Parodi and Ataúlfo Martínez-Torres
Chapter 16 Drug Screening and Drug Safety
Evaluation by Patch Clamp Technique 311
Yan Long and Zhiyuan Li
Chapter 17 Enhanced Patch-Clamp Technique to Study Antimicrobial
Peptides and Viroporins, Inserted in a Cell Plasma Membrane
with Fully Inactivated Endogenous Conductances 329
Marco Aquila, Mascia Benedusi, Alberto Milani and Giorgio Rispoli
Preface
This book deals with the understanding of endogenous mechanisms of cells and their
receptors as well as advantages of using this technique. It covers the basic principles
and preparation types and also deals with the latest developments in the traditional
patch clamp technique. Some chapters in this book take the technique to a next level of
modulation and novel approach.
The patch-clamp technique was developed by Neher and Sakmann in 1976 as an
effective refinement of the voltage-clamp-assay that demonstrated the function of
single ion channels in fundamental cellular process. The patch-clamp technique has
revolutionized the study of ion channels in a wide variety of cells.
This book has seventeen chapters and has been divided into two sections. First section
deals with the exploration of underlying mechanisms of cellular activities using patch
clamp technique. The second section deals with the advantages of this system.
The first section begins with a chapter on the black box properties of the system
followed by how intracellular signalling pathways integrate the pore associated to
P2X7 receptor with other large pores. Next chapter deals with combine usage of
whole-cell patch and focal stimulation technique to study of single synapse of central
nervous system. This is followed by the chapter on single-channel properties and
pharmacological characteristics of KATP channels in primary afferent neurons.
Chapters five and six explore how renal potassium channels are regulated by protein
kinases and phosphatases and characteristic features of BK channels respectively.
Then there are papers on "onion-peeling" technique, mitochondrial channels,
membrane transport in red blood cells and electrical properties in Leishmania-
macrophage membrane.
Seven chapters under section entitled “Advantages of using patch clamp technique”
describe how this electrophysiological method is found to be useful in studying
cardiac channelopathies, gating charge movements, off target effects of clinically used
drugs, Non-Steroidal Anti-Inflammatory Drugs (NSAIDs) in Modulating Vascular
Smooth Muscle Cells, Perforated Patch Clamp in non-neuronal cells, Drug screening
and drug safety evaluation and antimicrobial peptides and viroporins.
X Preface
This book will be of good value for students of physiology, neuroscience, cell biology
and biophysics.
Dr. Fatima Shad Kaneez
Professor Human Physilogy
PAP RSB Institute of Health Sciences
Universiti Brunei Darussalam
Brunei Darussalam
